Journal: The Kaohsiung Journal of Medical Sciences

Article Title: CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR ‐199b‐5p and regulating STRN expression

doi: 10.1002/kjm2.12631

Figure Lengend Snippet: Knockdown of circTNPO3 inhibited proliferation and invasion of HCC cells. (A) circTNPO3 expression in HL‐7702, Huh7, and HepG2 cells was detected by qRT‐PCR. sh‐circTNPO3 and OE‐circTNPO3 vectors are constructed and transfected into Huh7 and HepG2 cells. (B) circTNPO3 expression in Huh7 and HepG2 cells. (C) CCK‐8 measured cell viability of Huh7 and HepG2 cells at 24, 48, and 72 h. (D) Huh7 and HepG2 cell proliferation were assessed by EdU. (E) The apoptosis of Huh7 and HepG2 cells was detected by flow cytometry. (F) Transwell tested Huh7 and HepG2 cells invasion ability. (G) Western blot measured cleaved caspase3, bax, and bcl‐2 protein expression. The data were expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. HCC, hepatocellular carcinoma

Article Snippet: The primary antibodies including cleaved caspase3 (19677‐1‐AP, 1: 2000, Proteintech), bax (Ab32503, 1: 1000, Abcam), bcl‐2 (12789‐1‐AP, 1: 1000, Proteintech), STRN (21624‐1‐AP, 1: 2000, Proteintech) and GAPDH (10494‐1‐AP, 1: 5000, Proteintech) were incubated overnight at 4°C.

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Construct, Transfection, CCK-8 Assay, Flow Cytometry, Western Blot

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR ‐199b‐5p and regulating STRN expression

doi: 10.1002/kjm2.12631

Figure Lengend Snippet: Knockdown of miR‐199b‐5p reversed circTNPO3 silencing effect on HCC cells proliferation and apoptosis. miR‐199b‐5p inhibitor and sh‐circTNPO3 alone or co‐transfected into Huh7 and HepG2 cells. (A) qRT‐PCR detected miR‐199b‐5p expression in Huh7 and HepG2 cells. (B) CCK‐8 tested cell viability of Huh7 and HepG2 cells at 24, 48, and 72 h. (C) EdU detected Huh7 and HepG2 cells proliferation. (D) Flow cytometry measured Huh7 and HepG2 cells apoptosis. (E) Transwell examined Huh7 and HepG2 cells invasion ability. (F) Western blot assessed cleaved caspase3, bax, bcl‐2 protein expression. The data were expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. HCC, hepatocellular carcinoma

Article Snippet: The primary antibodies including cleaved caspase3 (19677‐1‐AP, 1: 2000, Proteintech), bax (Ab32503, 1: 1000, Abcam), bcl‐2 (12789‐1‐AP, 1: 1000, Proteintech), STRN (21624‐1‐AP, 1: 2000, Proteintech) and GAPDH (10494‐1‐AP, 1: 5000, Proteintech) were incubated overnight at 4°C.

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Western Blot

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR ‐199b‐5p and regulating STRN expression

doi: 10.1002/kjm2.12631

Figure Lengend Snippet: miR‐199b‐5p suppressed HCC progression by inhibiting STRN expression. (A) and (B) STRN mRNA and protein levels expression in Huh7 and HepG2 cells were tested by qRT‐PCR and Western blot. (C) CCK‐8 detected cell viability of Huh7 and HepG2 cells at 24, 48, and 72 h. (D) EdU measured Huh7 and HepG2 cells proliferation. (E) Huh7 and HepG2 cells apoptosis was detected by flow cytometry. (F) Huh7 and HepG2 cells invasion ability was detected by transwell. (G) Western blot tested cleaved caspase3, bax, and bcl‐2 protein expression. The data were expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. HCC, hepatocellular carcinoma

Article Snippet: The primary antibodies including cleaved caspase3 (19677‐1‐AP, 1: 2000, Proteintech), bax (Ab32503, 1: 1000, Abcam), bcl‐2 (12789‐1‐AP, 1: 1000, Proteintech), STRN (21624‐1‐AP, 1: 2000, Proteintech) and GAPDH (10494‐1‐AP, 1: 5000, Proteintech) were incubated overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Flow Cytometry